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TU-Tungt utstyr

Multichannel microarray reader

Awarded: NOK 0.97 mill.

The traditional approach employed to measure the RNA concentrations on microarray plates is to detect the fluoroscence intensities of two chromophores at single wavelengths (channel) only. Impurities on the plates often contaminate the results such that the estimation of RNA concentrations becomes inaccurate. Recent developments by Dr. Haaland at Sandia Laboratories in the USA clearly demonstrates significant improvement in sensitivity and accuracy of microarray experiments by using a multichannel reader/detector. A combination of multichannel data and chemometric data analysis was demonstrated by Dr. Haaland to be efficient in removing the effects of impurities and other spectral artifacts. To stay abreast with the fast developments in microarray technology funding for the construction of such a multichannel reader here at NTNU is requested. As these instruments are not yet commercially available it is necessary for us to make a prototype. Fortunately, NTNU has previous experience with construction of both a prototype microarray printing robot and a fluorescence scanner. The scanner was made both modular and extendable and will form the basis for the multichannel scanner.

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TU-Tungt utstyr

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