Back to search

BIOTEK2021-Bioteknologi for verdiskaping

Development of technology for flow cytometry- and other cell-based, high-content, high-throughput analyses to support marine bioprospecting

Awarded: NOK 4.0 mill.

Project Number:

208505

Application Type:

Project Period:

2011 - 2015

Subject Fields:

Partner countries:

ChemBioNet Norway - Norwegian Chemical Biology Platforms is a national network of HTS screening platforms (part of ESFRI-project EU-Openscreen) that support marine bioprospecting and drug screening. A joint application to the Infrastructure Programme for investments to support marine bioprospecting has been filed. Here staff and operation costs for development of technologies, research , competence building and training at the Oslo Node is requested. Technology development and research will particularly f ocus on cell-based assays for high content screening and looking at proliferation, apoptosis, secretion, cytokine production and importantly cell signaling networks in various cell types. Modulation of signaling events by natural compounds can be analysed by flow cytometry in single cells rather than by classical biochemical approaches which requires more material and is averaging the effect. Combining flow cytometry with a panel of phosphospecific antibodies against several signal molecules simultaneousl y in a multi-parameter analysis enables a higher level of understanding of the effect of compounds on signal transduction dynamics at a single cell level. In addition, analysis of mixed cell populations makes it possible to study cells ex vivo in a state more closely resembling the in vivo situation. Furthermore, fluorescent cell barcoding (FCB) labeling allows pooling of samples such as e.g. time courses or concentration curves in a single flow run. We will develop this methodology for marine bioprospect ing and drug screening as a strategy that will directly address the effects on human primary cells (e.g. immune cells) and avoid some of the caveats integrated into current functional genomics screening approaches that are based on cell lines and recombin ant proteins. We have already established flow cytometry-based medium-throughput and will next reach to HTS of active compound libraries in model systems for inflammation, cancer and immune therapy.

Publications from Cristin

No publications found

No publications found

No publications found

No publications found

Funding scheme:

BIOTEK2021-Bioteknologi for verdiskaping