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BIOTEK2021-Bioteknologi for verdiskaping

DL: OXYMOD - Optimized oxidative enzyme systems for efficient conversion of lignocellulose to valuable products

Alternative title: OXYMOD - Optimaliserte redoks-enzymsystemer for effektiv bearbeiding av lignocellulose

Awarded: NOK 20.0 mill.

Project Number:

269408

Application Type:

Project Period:

2017 - 2022

Location:

Subject Fields:

Partner countries:

In the OXYMOD project scientists with different competences work on discovering, understanding and further developing enzyme systems for the conversion of lignocellulose from Norwegian biomass into valuable products like sugars and aromatic chemicals. Redox enzymes have huge potential in the depolymerisation of cellulose, hemicellulose and lignin, but studying these enzymes is difficult and requires a systems approach. Thus, OXYMOD goes further than basic enzyme discovery, as we study the interplay between redox enzymes, their co-factors and their reaction partners, as well as the interplay between these novel enzymes and hydrolytic enzymes. OXYMOD studies these enzyme systems by looking at approx. 1000 marine Actinobacteria isolates whose genomes have been sequenced. OXYMOD combines life sciences (enzyme biochemistry, enzyme production technology, microbial biotechnology, high throughput screening, advanced analytics), ICT (bioinformatics, big data handling), mathematical sciences (enzyme systems and process modelling) and engineering (enzyme evolution, synthetic biology) for producing new biocatalytic systems for industrial application. Our focus is on developing enzyme solutions for the agricultural and forest sectors. Besides the enzymes and enzyme systems themselves, innovations from OXYMOD concern the generation of well-defined products streams, primarily sugars from (hemi-)cellulose and aromatic building blocks from lignin, for downstream applications (e.g. biofuels, bioplastics, feed). Additional applications can be envisaged, such as the development of green (= enzymatic) methods for producing fine chemicals. We have carried out large bioinformatic analyses to find enzyme candidates, which have been produced and characterized. A lot of energy was put into getting the enzymes produced, which is done by e.g. the company Vectron, who has managed to develop a new system for protein production in Streptomyces that is unique to Norway and very useful for several projects. We work with enzymes that in themselves have interesting applications and here we have made great progress (two PNAS papers). Based on these results, we are currently studying complete enzyme systems, i.e., mixtures of interacting enzymes, as they exist in nature. An unexpected amount of energy had to be used to study so-called abiotic factors (e.g., the presence of metal ions, redox active small molecules, and oxygen), because the project has shown that these factors have major effects on the efficiency of the enzyme systems. In order to understand and model our enzyme systems, the properties of the enzymes must be analyzed very carefully; therefore, we carry out a number of advanced studies of structure, dynamics and function (kinetics), partly in collaboration with our Portuguese project partners. We have a close dialogue with several interested companies and innovation experts from both Digital Life and NMBU. Both participate regularly in the project meetings. The project has an active RRI component, and a PhD student has submitted his dissertation that is based, in part, on RRI work in the project. Important progress from 2021 includes: - Thesis on RRI submitted: ?A philosophical interpretation of the development of neo-Schumpeterian theory and policy?. - Strong collaboration with a Portuguese partner (UNL) on characterization of LPMOs (work in progress, paper in 2022) a Dyp type peroxidase (paper published) and MCO (work in progress). With NMBU and SINTEF. - Strong collaboration with the other Portuguese partner (LNEG) on fractionation and characterization of biomass; purification of lignin; at least one joint publication in 2022. With NTNU, NMBU and SINTEF. There is a connection between lignin work at LNEG and work on the impact of light on biomass conversion, which is done in another project at NMBU. - Enzyme expression in Streptomyces, developed at Vectron. With NTNU and SINTEF. - Deep insight into how LPMOs work, including the impact of abiotic factors and of so-called Carbohydrate-Binding Modules (CBMs) appended to the enzymes. Several articles published or in press. NMBU, NTNU, SINTEF. - Based on this insight: first steps toward assembling and optimizing (synthetic) redox enzyme systems for biomass conversion. NMBU, SINTEF, NTNU. - Experimental characterization of laccases and peroxidases, linked to modeling studies. SINTEF (with UNL). One paper published, another paper in progress. - Protein engineering of LPMOs, laccases and Dyp-type peroxidases is in progress. (SINTEF, NMBU; med UNL). One paper published in 2021. - "High-throughput" screening of the biomass degrading properties of a unique collection of microorganisms at SINTEF, including the development of new rapid screening methods has led to the identification of strains with very interesting properties, such as the ability to convert and degrade lignin. These strains are currently used for proteomics-based enzyme discovery.

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The OXYMOD project will through a transdisciplinary approach define, develop and demonstrate applicability of new enzyme systems for the efficient biocatalytic conversion of lignocellulose from abundant Norwegian biomass into valuable products like sugars and aromatic building blocks. OXYMOD will focus on the still largely underexplored group of redox enzymes and their potential in the depolymerisation of cellulose, hemicellulose and lignin, including aspects such as redox enzyme interplay, co-factors and reaction partners, as well as their interplay with hydrolytic enzymes. OXYMOD will address these enzymes and enzyme systems as they occur and function in, among others, a unique in-house collection of approx. 1000 marine Actinobacteria isolates with genomes recently sequenced. Redox enzymes require co-factors and redox partners, and there is a considerable degree of cooperativity between different enzyme classes. Enzyme systems-scale understanding and eventually engineering the efficient degradation of lignocellulose by these enzyme systems, requires an integrated transdisciplinary approach far beyond 'simple' enzyme discovery. OXYMOD combines life sciences (enzyme biochemistry, enzyme production technology, microbial biotechnology, high throughput screening, advanced analytics), ICT (bioinformatics, big data handling), mathematical sciences (enzyme systems modelling, process modelling) and engineering (enzyme evolution, synthetic biology) for producing new and optimized biocatalytic systems for industrial application, primarily within the agricultural and forest sectors. Besides the enzymes and enzyme systems themselves, additional innovations from OXYMOD concern the generation of well-defined products streams, primarily sugars from (hemi-)cellulose and aromatic building blocks from lignin for a variety of downstream applications (e.g. biofuels & bioplastics).

Publications from Cristin

No publications found

Funding scheme:

BIOTEK2021-Bioteknologi for verdiskaping

Thematic Areas and Topics

LTP2 HavBransjer og næringerProsess- og foredlingsindustriGrunnforskningPolitikk- og forvaltningsområderFiskeri og kystBioteknologiMiljø og naturmangfoldLTP2 Bioøkonomi og forvaltningNordområdeneMiljø og naturmangfoldMiljøteknologiInternasjonaliseringEtiske, juridiske og samfunnsmessige aspekterDelportefølje Sirkulær økonomiPortefølje LivsvitenskapLTP2 Styrket konkurransekraft og innovasjonsevneLTP2 Klima, miljø og miljøvennlig energiDelportefølje Klima og miljøMiljøteknologiAnnen miljøteknologiIKTIKT - Bruk og anvendelser i andre fagLTP2 Nano-, bioteknologi og teknologikonvergensLTP2 Marine bioressurser og havforvaltningLTP2 Utvikle fagmiljøer av fremragende kvalitetLTP2 Et kunnskapsintensivt næringsliv i hele landetBioteknologiLandbruksbioteknologiIKTPortefølje Industri og tjenestenæringerLTP2 Klima, polar og miljøBransjer og næringerPolitikk- og forvaltningsområderNæring og handelAvanserte produksjonsprosesserAvansert produksjonsteknologi som fag og teknologi (ny fra 2015)MiljøteknologiIKTIKT - Som fag og teknologiBioøkonomiLTP2 Muliggjørende og industrielle teknologierPolitikk- og forvaltningsområderSkog, landbruk og matMarinLTP2 Fagmiljøer og talenterNordområdeneKlima, miljø og biologiske ressurserInternasjonaliseringInternasjonalt prosjektsamarbeidPortefølje Landbasert mat, miljø og bioressurserLTP2 IKT og digital transformasjonMarinMarin bioteknologiBioteknologiIndustriell bioteknologiBioteknologiMarin bioteknologiPolitikk- og forvaltningsområderPortefølje Muliggjørende teknologierAvanserte produksjonsprosesserMiljø og naturmangfoldSirkulær økonomiPortefølje HavSirkulær økonomi