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BIOTEK2021-Bioteknologi for verdiskaping

ERA-NET: Secondary bioprod. of low trophic organisms utilizing side streams from Blue & Green sectors to prod. feed ingredients -SIDESTREAM

Alternative title: SIDESTREAM

Awarded: NOK 7.2 mill.

The goal of the SIDESTREAM project was to evaluate new ways of recycling aquaculture and agriculture wastes to save resources and create value. Aquaculture and agriculture produce vast amounts of wastes. The SIDESTREAM project regards these wastes as 'side streams' and considers them a resource. We investigated to which degree marine organisms such as polychaete worms and gammarid shrimps, and also bacteria can be utilised to recapture nutrients, energy and biochemicals from side streams. Furthermore, we evaluated the suitability of the biomass as a fish and shrimp feed ingredient. Finally, we evaluated the sustainability and economic feasibility of these activities. All side streams used as feed input for the projects model species were utilized as feeds, in some cases (e.g., liquid phase biogas side streams for bacteria) pre-treatments were necessary. Rearing protocols for the marine polychaete Hediste diversicolor, the gammarid shrimp Gammarus locusta, and the genetically modified Corynebacterium glutamicum have been established. These protocols have been developed to optimize biomass production, resource utilization and production of high nutritional value fatty acids (gammarids and polychaetes), and astaxanthin (C. glutamicum). During the project we learned how to treat these biomasses to process and stabilize valuable compounds. Further, polychaete, gammarid and bacterial meals were formulated into fish and shrimp feed and tested for their suitability as replacement of either fish meal or fish oil, or as attractants. Juvenile sea bass (Dicentrachus labrax) thrived on diets replacing 10%, 20%, or 40% of the fish meal with polychaete meals. None of the fish fed polychaete meals differed in growth, feed conversion, or protein digestion efficiency from those fed commercial sea bass diets. Juvenile rainbow trout fed diets containing astaxanthin produced within the project showed less appetite for the diets compared to trout fed diets containing commercially available astaxanthin sources, which was also reflected in reduced growth rates. However, it shall be noted that the astaxanthin produced in the project was used as autoclaved bacterial biomass, not as extracted pigments, which clearly differs from using synthetically produced astaxanthin, and the result is hence to be treated with caution. LCA analysis revealed that electricity and water usage is a main driver of costs and decreased sustainability when biogas digestate shall be used as feed input. The reason for this is the high ammonia content which needs to be removed from the solid phase. LCC comparing a high-tech and a low-tech scenario revealed that scaling effects in the low-tech scenario by far exceeds those in high-tech scenarios. LCA on polychaete meals as a fish meal replacer revealed that suggests that the circular approach of rearing polychaete on aquaculture wastes activities may reduce the environmental impact by 23% as opposed to a linear approach. SIDESTREAM covered several steps along the value chain – from biomass production to the provision of products through conversion and processing. SIDESTREAM enhanced our knowledge on the ability of invertebrates to produce high value compounds. The project led to novel and refined production techniques and protocols leading to increased production of these compounds under highly controlled conditions. Up-to-date information about the project can be found on the project website:

The main impact of SIDESTREAM is the creation of value from material hitherto considered side stream, creating solutions for waste problems by offering new, highly sought-after products such as feed ingredients and high value biochemicals. Project results suggest that the SIDESTREAM approach is feasible and next steps to commercialisation can be taken.

To which extent is it possible to recycle nutrients from aquaculture and agriculture waste by secondary bio-production? Which organisms and approaches are best suited? Can these organisms serve as ingredients for feed stuff? How safe are feed ingredients produced on waste? What is the market potential and economic feasibility of feed ingredients produced following sustainable circular principles? SIDESTREAM addresses these questions to push forward the frontier for production of high value compounds by utilization of low trophic marine invertebrates and bacteria, which will be reared on waste streams following circular principles. Several industries are in demand for high value compounds such as marine-originated lipids, proteins and pigments such as astaxanthin. Omega-3 long-chain (=C20) polyunsaturated fatty acids (?3 LC-PUFA) are marine lipids that are abundant in fish oils and fish meals (“marine ingredients”). ?3 LC-PUFA in aquafeeds ensures both growth and health of farmed species and their nutritional value for consumers. Aquaculture is by far the largest consumer of marine ingredients thus requiring novel high nutritional quality ingredients to critically ensure sustainable expansion. Additionally, there is a trend towards natural bioactive compounds such as astaxanthin as a natural bioactive stimulant (nutraceutical), which adds value to the resulting feed. SIDESTREAM partners have shown that polychaetes and crustaceans can produce ?3 LC-PUFA de novo. Such striking capacity will be exploited in SIDESTREAM. We will take side streams from aquaculture, agriculture and biogas sectors, and produce polychaetes and gammarids on the solid phase and bacterial astaxanthin on the liquid phase. Biomasses will be processed and tested as feed ingredients for fish and shrimp during all life stages. SIDESTREAM cutting-edge approach will enable value creation from resources hitherto considered as waste, allowing for innovation and sustainable use in aquaculture.

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BIOTEK2021-Bioteknologi for verdiskaping

Thematic Areas and Topics

BioteknologiIndustriell bioteknologiLTP3 Samfunnssikkerhet og beredskapLTP3 Samfunnsikkerhet, sårbarhet og konfliktBransjer og næringerNæringsmiddelindustriNordområdeneKlima, miljø og biologiske ressurserMatMat - BlågrønnMatGlobal matsikkerhetMatMat - Blå sektorPolitikk- og forvaltningsområderFiskeri og kystMatNæringsmiddel og foredlingMatMat - Grønn sektorPolitikk- og forvaltningsområderSkog, landbruk og matPolitikk- og forvaltningsområderMiljø, klima og naturforvaltningLTP3 Fagmiljøer og talenterNordområdenePortefølje InnovasjonResponsible Research & InnovationRRI Utviklings- og prosessorienteringHavbrukFôr og ernæringPolitikk- og forvaltningsområderResponsible Research & InnovationMarinMarin bioteknologiGrunnforskningLTP3 Hav og kystAnvendt forskningPortefølje Banebrytende forskningBioøkonomiMarinBioteknologiLandbruksbioteknologiBransjer og næringerFiskeri og havbrukBioøkonomiØvrig bioøkonomiCo-Funded/ERA-NETBioteknologiMarin bioteknologiLTP3 Et kunnskapsintensivt næringsliv i hele landetBransjer og næringerInternasjonaliseringHavbrukLTP3 Klima, polar og miljøBransjer og næringerLandbrukInternasjonaliseringInternasjonalt samarbeid om utlysningLTP3 Marine bioressurser og havforvaltningNaturmangfold og miljøCo-Funded/ERA-NETERA-NET Cofund H2020LTP3 Høy kvalitet og tilgjengelighetMarinHavbrukPortefølje ForskningssystemetLTP3 Bioøkonomi og forvaltningLTP3 Muliggjørende og industrielle teknologierBioteknologiPortefølje Mat og bioressurserLTP3 Klima, miljø og energiLTP3 Styrket konkurransekraft og innovasjonsevneBioøkonomiSektorovergripende bioøkonomiLTP3 Nano-, bioteknologi og teknologikonvergensMatInternasjonaliseringInternasjonalt prosjektsamarbeidPortefølje Klima og miljøNaturmangfold og miljøSirkulær økonomiKlimarelevant forskningPortefølje Muliggjørende teknologierLTP3 Rettede internasjonaliseringstiltakSirkulær økonomi