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BIOBIOT-Biologi, bioteknologi, Mat. Nat.

The biological role of catalytic RNAs

Tildelt: kr 1,4 mill.

We have discovered and characterized a new category of group I introns called the twin-ribozyme introns. The complex organizations of these introns reflect a dual biological role in intron splicing and expression of proteins from nucleolar DNA of eukaryot ic cells. A fundamental and general question is how the intron-encoded proteins are expressed from RNA polymerase I transcripts -which is "off the rule" among eukaryotes. We have previously reported that the expression homing endonucleases in the eukaryot ic twin-ribozyme introns Dir.S956 and Na.S516 are dependent on a novel class of ribozymes (the GIR1's), removal of a spliceosomal mini-intron, and poly adenylation in orde r to make a functional processed messenger RNA (EMBO J. 18: 1003-1019, 1999; RNA6: 616-627, 2000). This project focuses on additional essential features in biological function of catalytic RNAs in Dir.S956-1 and Na.S56<16: RNA-RNA tertiary interactions as dynamic structures. One tertiary base-pairing interaction (Na.S516) and three tertiary GNRA tetra loop-helix interactions (Dir.S956-1 and Na.S516) will be characterized in vitro and in vivo (E. coli and yeast), by evaluating the expression patterns of intron-encoded endonucleases and retrohoming.

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BIOBIOT-Biologi, bioteknologi, Mat. Nat.

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