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BIOPROSP-Bioprospektering 2002 - 2006

Bioactive substances from Norwegian Aquatic micro-organisms as cell signalling disruptors with potential to modulate cell death and thromboc

Tildelt: kr 13,1 mill.

Bioactive substances from Norwegian Aquatic micro-organisms as cell signalling disruptors with potential to modulate cell death and thrombocyte activation I. Isolation of about 50 new microbes from unusual aquatic habitats. II. Moderate biomass production from above novel microbes and from 500 other aquatic microbes selected from collections at UoO, NIVA, UoB and the MRI, particularly isolates from benthic habitats and from living macro-organisms. III. Chemical extraction (water, 1/1 water/methanol, 1/1 methanol/di-Cl-methane) of above biomass samples IV. Screening by microscopy for apoptogenic activity against leukemia cells, T-cells, hepatocytes. Screening by HPLC analysis of thrombocyte inhibition. V. Purification (mainly by HPLC) of bioactivity. Elucidation of structure by MS (Q-TOF) and NMR. VI. Elucidation of mechanism of action using cells with defined defects in apoptotic signalling, proteomics, assays of selected protein kinases and biocomputational docking. VII. Test for antileukemic and anti-thrombotic effects in mice. VIII. Patent and commercialize compounds IX. Characterize genetically the microbes producing interesting bioactive substances, and search for genetic features that may predict the exploitation potential of novel isolates.

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BIOPROSP-Bioprospektering 2002 - 2006

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