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BIOPROSP-Bioprospektering 2002 - 2006

Natural gas and biotechnology: bioprospecting of methane-oxidizing bacteria (NABI).

Tildelt: kr 6,7 mill.

Methanotrophic bacteria are widespread in nature and can grow on methane, the major component of natural gas, as the sole source of carbon and energy. As one of the world's major producers of natural gas, Norway has a great potential for developing an ext ensive industry based on the use of natural gas in large-scale production of bioprotein and biochemicals by methanotrophs. A large-scale unit for production of single-cell protein is already established, and the bioprotein has been licensed as feed for fi sh and animals. We propose to perform a large-scale screening of methanotrophs in a variety of methane-containing environments such as methane seeps on the ocean floor, hot springs at Spitsbergen, gas-fields in the North Sea, and soil and tropical rice-fi elds. The aim is to provide strains with improved properties for fermentation of crude natural gas, genetic elements for development of tools for genetic manipulation, and strains that produce bioactive substances such as bacteriocins and antibiotics. Organisms will be enriched and isolated under a wide range of cultural conditions and screened for a variety of biological properties. An automated robotic system with capacity of picking more than 30,000 colonies per day will be utilized in a high-throughput screening program.

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BIOPROSP-Bioprospektering 2002 - 2006

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