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TU-Tungt utstyr

Utstyr for Protein-protein samhandling i cellesignal

Tildelt: kr 1,7 mill.

Anchored and scaffolded signalling pathways involving kinases and phosphatases are implicated in major chronic diseases such as HIV, cancer, diabetes type II, cardiovascular disease, and also underpins various genetic diseases. However, no systematic app roach has explored the therapeutic potential in tergeting and disrupting anchored signal pathways in conditions such as listed above with a dysregulated signal pathway. The focus on the research in the present proposal is to devise and test of therapeutic stra tegies based on understanding function of anchored signaling netowrks, the down-stream effectors, interaction with targeting molecules, and substrates. Specifically, this involves building a facility with instruments for screening, mapping and affinity analysis of prote in-protein interactions in cell signaling with "state-of-the-art" techiques such as peptide libary mapping, in-solution interaction, and solid phase interaction. Peptide synthesizer with spotter will allow walking through proteins for mapping of interaction domains and making libraries for identification of specificity and affinity. The Fusion alpha instrument with pipetting robot Multiprobe II will allow for in-solution screening and fluorescence polarization and is suitable for screening protein-protein interactions in medium and high-throughput and exploring ligand protein interactions in signaling and drug development. Surface plasmon resonance (BiaCore, appl. for year 2, 2004-5) will allow precise characterization of affinities.

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TU-Tungt utstyr

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