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BIOMOL-Molekylær biovitenskap og bioteknologi

Genetic dissection of maternal effects in early embryo and endosperm development

Tildelt: kr 4,2 mill.

Seed development is an interplay of different tissues that execute different transcriptional programmes. By double fertilization two haploid sperm cells fuse with the haploid egg cell and the diploid central cell of the female gametophyte to produce the d iploid embryo and the triploid endosperm, respectively. Seed development is the result of the concerted action of the embryo and endosperm developmental transcriptional programs. In addition, factors produced in the diploid maternal tissue influence these programs. Recently, maternal factors produced in the haploid gametophyte (egg cell and central cell) also have been hypothesized to play a vital role in this process. Whereas hundreds of genes vital for embryo and endosperm development have been identifi ed over the past few years, only a handful of genes showing a maternal effect on embryo and endosperm development have been characterized.The aim of this project is to study the action of early transcriptional programmes active in different seed tissues m aking use of forward genetics, reverse genetics and transcriptional profiling. The common basis for these studies is the use of mutants obtained by reverse or direct screens, with a strong focus on mutants or genes that exhibit gametophytic maternal effec ts.

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BIOMOL-Molekylær biovitenskap og bioteknologi

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