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MATPROG-Matprogrammet: Norsk mat fra sjø og land

NKJ 125 Torward a standard for microarray-based characterization os Salmonella isolates: A novel tool in risk assessments

Tildelt: kr 0,75 mill.

Prosjektleder:

Prosjektnummer:

175687

Søknadstype:

Prosjektperiode:

2006 - 2009

Geografi:

8. Summary of the project plan (background, research methods, goals, timetable): Salmonella-induced enterocolitis in humans is an important food-safety issue. Efforts continue to assess the genomic variation among environmental, veterinary, and clinical i solates in order to trace the infections and support the risk assessments and intervention strategies. Microarray technology is an emerging diagnostic tool, which could be useful for rationalized characterization of several thousand isolates at a time. Ho wever, several international working groups (www.foodpcr.com) have identified standardization of DNA microarray analysis as one of the urgent needs, before it can be used as a consensus method across boundaries. The overall aim is to provide risk managers with an additional tool for Salmonella control. The specific goal is to study the usefulness of three microarray platforms for a comprehensive development of a diagnostic DNA microarray for the characterization of Salmonella isolates in the Nordic countr ies. The partners have access to fully equipped microarray laboratories and have worked with Salmonella array for some time. The aspects of discriminatory power, robustness, reproducibility, cost-effectiveness, ease-of-use, etc. will be evaluated in colla borative ring-trials. The standard protocol will facilitate comparison of a large number of Salmonella isolates using markers for approx. 200 important genes. Substantial work is needed with sequence information and statistical data analysis. This will pr ovide a novel and rationalized tool in risk assessment and risk management, which is needed for intervention strategies in risk management. A Ph.D. student will work for 36 months at the partner institutes. International collaboration is included through active participation of in the European network of Excellence MedVetNet (www.medvetnet.org) and collaboration with Dr. Burkhard Malorny from BfR, Berlin; Dr. Muna Anjum, VLA, UK, and Dr. Kris Rahn, from Canada (see letters enclosed). The work proposed wil l supplement similar European activities at the partners’ laboratories. The Ph.D. student will also visit the US and Canadian laboratories for exchange of technologies and protocols. The project will include a number of external partners in the EU as part icipants. A training workshop for end-users will be organized during the project. The work is intended to produce a draft protocol for NMKL standard.

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MATPROG-Matprogrammet: Norsk mat fra sjø og land