Tilbake til søkeresultatene


Application of monolithic columns in algal virus research - Sandra Kolundzija - Croatia

Tildelt: kr 42 000

Although HPLC (High Performance Liquid Chromatography) is widely used separation method, its application using conventional (particle based) stationary phases, is rather restricted to smaller molecules. However, by using monolithic chromatographic support s, it enables processing of large biomolecules, even macromolecular complexes, such as virus particles. Moreover, the structure of monoliths enables very fast separation and the process is scalable to large volumes. So far, different viruses were separate d/purified from different media and concentrated from large-volume water samples. By exploiting complementary experience in both monolith chromatography and marine virology, a bilateral project proposed by the University of Zagreb and the University of Be rgen would be an implementation of the powerful separation technique in very important and attractive field of marine biology. Following selection and preparation of suitable algal virus/host systems, monolith chromatography would be optimized and proved in fast and efficient purification of cultivated algal viruses, fast and reliable quantification of viruses propagated in algal host cultures, and possible alternative/complementary approach in assessment of viral diversity and abundance concerning domina te virus species present in environmental samples. The results would be evaluated by comparing to those obtained using standard methods established so far.