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Limb wounds in horses are common, however healing is exceedingly slow and fraught with complications. Contrary to body wounds, limb wounds demonstrate only a small amount of wound contraction. The specialist procedure of applying split-thickness skin grafts to limb wounds stimulates wound contraction by unknown mechanisms. The objective of this project is to identify those mechanisms to develop a treatment strategy for equine limb wounds for use in general  practice. Limb wounds are severely impacting animal welfare due to an extended healing/treatment duration, and the risk of euthanasia due to a poor functional outcome. By stimulating wound contraction, healing time will be reduced, and the functional outcome improved, directly benefitting the welfare of wounded horse and reducing treatment cost for the owner. Aim: To identify dermal components stimulating wound contraction, and to develop this knowledge into future state-of-the-art treatment of equine limb wounds for use in general practice. Hypotheses: i) Application of dermal fractions to collagen gels seeded with equine fibroblasts will increase gel contraction in vitro; ii) Application of dermal fractions to experimental limb wounds in vivo, will increase wound contraction resulting in a faster healing time versus wounds treated by conventional methods. Methods: i) The contractile effect of different dermal components will be assessed in a collagen gel/ fibroblast model; ii) Identified dermal components will be tested in a standardized experimental limb wound model in vivo (n=5). Ultimately, results from this project are expected to improve wound treatment in horses, directly impacting animal welfare by reducing healing time and improving the functional outcome of wounded horses. This will also directly benefit horse owners and caretakers and making veterinarians able to offer better treatment solutions.

HESTEFORSK - Raskere og bedre sårheling hos hest med autologe hudkomponenter

The project aims to develop a high-fidelity MVP of robotic furniture designed to optimize urban living spaces and enhance life quality. This initiative merges advanced robotics and AI with practical design to address space efficiency in densely populated areas.

Key R&D challenges include integrating robotics and AI to create adaptable furniture systems, ensuring safety through rigorous testing and third-party certifications, and applying real-world feedback to refine functionality. The project involves significant technical and regulatory hurdles in engineering consumer-friendly, safe, and responsive furniture.

Starting December 2024, a three-month pilot phase with selected Norwegian businesses will test the product's real-world application and collect operational data. Feedback from end-users will be gathered through qualitative interviews, informing iterative improvements to enhance design and functionality.

This project leverages existing scientific, technical, and business knowledge in innovative ways, aiming to develop a unique product that sets new industry standards. It is characterized as experimental development, pushing technological boundaries in furniture design.

By February 2025, feedback and pilot data will be used to finalize a market-ready product, ensuring it meets user needs for functionality and aesthetics. This initiative represents a pioneering effort to transform urban living, combining experimental development with practical application to create sustainable and adaptable living solutions.

Development of high-fidelity MVP of AI driven robotic furniture to be piloted in realistic environment with pilot clients.

I will carry out this fellowship at the University of Oslo, under the supervision of Professor Kenneth Karlsen at the Department of Mathematics.  The project is devoted to study existence and blow-up properties of the solutions of initial value problems (IVPs) for nonlocal nonlinear integrable PDEs. Nonlocal integrable equations were introduced by M. Ablowitz and Z. Musslimani in 2013 and have become a hot topic in the theory of integrable systems. By applying inverse scattering transform (IST) method it was shown that soliton solutions of nonlocal equations blow-up in isolated points in x,t plane. Therefore the natural question is to study the existence and blow-up properties of the general IVPs for such equations. To this end we will use the mixture of the IST method and PDE techniques. The former will be used for predicting the presence and the form of the blow-up of the solution, while the PDE approaches will be used for describing the blow-up of the solution of the general IVP and for finding a suitable concept of a weak solution, which exists even after the collapse.  Also by the IST method it was shown that apart from zeros, which correspond to solitons, the associated to the initial data spectral functions have the new type of singularities. These singularities correspond to the winding of the argument of the spectral function and play a significant role in long-time asymptotic behavior of the solutions ? they are responsible for a transition between qualitatively different asymptotic zones in x,t plane. It is a challenging open problem to describe analytically the nonlinear effects corresponding to these new singularities and in the framework of the project we are going to investigate this phenomena numerically.  The fellowship will allow me to expand my skills in PDE methods, which is different, but related to my present expertise field. Also it will enable me to become an established interdisciplinary researcher, who could pursue top academic positions.

Nonlocal integrable equations: blow-up phenomena and beyond

Renewable energy on the high seas – science-informed and indigenous knowledge-based recommendations for regulatory and governance challenges

The high seas are those marine areas under no State's jurisdiction. So far, all marine renewable energy (RE) activity is being conducted under State's jurisdiction. However, recent advances in technology are raising the prospect of carrying out RE activity on the high seas. Currently, only seabed mining activities in the Area have been regulated. The Area is the seabed and ocean floor beyond the limits of State's jurisdiction. From the legal viewpoint, the main gap is a lack of an international regulatory framework, informed by participatory mechanisms and supported by scientific evidence, for RE activities on the high seas, especially regarding their environmental and social impacts.  The primary project objective (O) is to provide the scientific community and policymakers with science-informed and indigenous knowledge-based recommendations in support of the development of  RE regulation on the high seas.  Two specific Os are set to complement the primary O, namely: O1 To advance the use of systematic consultation processes that facilitates research at the crossroads of marine sciences and law.  O2 To advance critical knowledge and understanding of environmental pressing issues comparable to the regulation of RE on the high seas, such as deep-sea mining in the Area. A multistep innovative methodology will be adopted, by integrating marine scientific knowledge, consultative processes, and advanced critical research in the subject matter. A case study (interdisciplinary and participatory) involving marine scientists, indigenous and coastal communities and policy makers will be conducted onboard the Research Vessel KronPrins Haakon at the beginning of the project. The case study outcomes will be analysed through critical legal analysis to build a knowledge base for policy recommendations.  The project will advance the PI’s career in an international research environment, allowing her to generate independent, high-quality, and reproducible results.   

BOLD MINDS aspires to enhance the understanding of vulnerability factors underlying the emergence of borderline personality disorder (BPD), with an ultimate aim of increasing the potential for young individuals to receive early, evidence-based help when their mental health is at risk. BOLD MINDS will achieve this by investigating whether disruptions in two aspects of autobiographical memory (AM), narrative identity (NI) and involuntary AM (IAM), are associated with BPD features and problem behaviours in adolescents concurrently and over time. In doing so, BOLD MINDS will strengthen the understanding of mechanisms underlying the emergence of BPD in adolescence and lay a groundwork for developing evidence-based early interventions for BPD. While currently limited, the study of AM in this field is essential for two reasons. Firstly, because the aspects of AM under investigation, NI and IAM, are known to be involved in identity formation and emotion regulation which represent two key psychological processes that are disrupted in adults with BPD and are linked to problem behaviours such as self-harm and suicidality. Secondly, because promising research is emerging on the use of AM targets in the treatment of emotional disorder in adults, and provides a strong model for future treatment development with vulnerable adolescents. In addition, this timely research program aligns with calls from international organizations to prioritize early interventions to promote adolescent mental health. BOLD MINDS will adopt and adapt state-of-the-art ecological momentary assessment and analytical techniques and work at the crossroads of clinical and cognitive psychological perspectives, allowing for a multidimensional approach to understanding BPD vulnerability. Training-through-research and formalized training of the experienced researcher will prepare her for a permanent academic position and the founding of her own research lab specializing in AM and early interventions for BPD.

Borderline Personality Disorder: Memory and Identity in Adolescence

Multilingual Acquisition and Processing: Heritage Russian in Spain

Recent research highlights a need of deeper understanding of the nature of language competence and the development of this competence under heritage language bi- and multilingualism. Heritage speakers (HSs) is a population highly diverse in terms of their linguistic competence and its development. Multilingual Acquisition and Processing (MAP): Heritage Russian in Spain will provide an examination of Russian Heritage Language (HL) grammar in a previously unstudied socio-linguistic context focusing in particular on the patterns of cross-linguistic interaction at early and most unstable stages of multilingual language development. The overall research objective is to discover the patterns of language acquisition and language processing in order to find out how multilingual minds deploy their developing grammatical knowledge and how they detect and access the linguistic (morpho-syntactic) mechanisms from their two co-existing languages in real-time. The proposed empirical study will investigate the effects of linguistic proximity, individual experiential factors and the developmental patterns of cross-linguistic influence in child bilingualism. Heritage Russian in Spain will be studied with a focus on 3-7-year-old children in order to examine developmental trajectories in both languages. On the theoretical level, MAP will significantly contribute to the development of a model of heritage language grammar and add to a sub-field of multilingualism studies, Heritage Language Acquisition, by showing the patterns of cross-linguistic interaction at early stages of multilingual Russian-Spanish development. On a societal and education level, project findings will be relevant for policy makers, teachers, school principals and HSs communities in European countries. To date, most heritage language studies have focused exclusively on the minority language, MAP will fill an important gap by focusing on both languages.

Algebraic Theory of Information Quantities

Information theory is a discipline at the intersection of statistics, engineering and computer science. As the study of information quantities, such as compression or communication capacities, information content or measures of statistical dependency, it is one of the theoretical underpinnings of data science.  Computational problems in information theory are highly non-linear. The goal of this project is to transfer state-of-the-art methods of computational non-linear algebra to information theory, to study the inherent algebraic complexity of information-theoretical problems and to provide tools for solving them in practice. The algebraic point of view has proven to be fruitful in seemingly unrelated areas, as witnessed by a surge of recent work in algebraic statistics, in particular on likelihood geometry. However, maximizing the likelihood function is the same as minimizing relative entropy — a specific information quantity. Hence, this project also aims at generalizing the techniques developed in likelihood geometry.  One focus is on the practical computation of information quantities using numerical and differential algebraic geometry. Such quantities are defined via non-linear optimization problems and we aim to pinpoint the algebraic complexity of these problems in instances of general interest, such as common information measures. The final objective is finding fundamental laws and limits of data science imposed by non-linear inequalities constraining the entropic region. These inequalities provide, by duality, universal bounds for many of the optimization problems studied in information theory.

Create EU & US Go-to-Market strategy (€35K). Work with two consultants in defining strategies.

Empire AI Women TechEU

Tenk på universet som en enorm ballong som alltid utvider seg. Jo lenger unna et punkt er på ballongen, jo raskere beveger det seg bort fra deg. Dette ligner på hvordan universet utvider seg, jo lenger unna en galakse er, jo raskere beveger den seg bort fra oss på jorden.

I mange år trodde forskerne at universets ekspansjon ble bremset. Begrunnelsen deres var at gravitasjonskraften fra all materie i universet virket mot denne utvidelsen. Imidlertid gjorde kosmologer i 1998 en overraskende oppdagelse gjennom studiet av fjerne supernovaer: utvidelsen av universet akselererer faktisk!

Dette betyr at avstanden mellom galaksene blir større og større i stadig økende hastighet. Det er som om ballongen blåses opp raskere og raskere.

Forskere vet ikke nøyaktig hva som får universet til å akselerere. De kaller den ukjente kraften som får dette til å skje "mørk energi". Mørk energi er overalt i universet, men den mørke energien vekselvirker ikke med materie i de formene som vi kan observere direkte.

NEVO-prosjektet er et nytt forsøk på å forstå egenskapene til mørk energi. NEVO vil bruke kraftige datamaskiner for å simulere universet og studere effekten av kandidater til den mørke energien på en måte som aldri har vært gjort før. Ved å studere disse simuleringene og utnytte høypresisjons-observasjonene fra Euclid-teleskopet som nylig er skutt opp i rommet, vil kosmologene være i stand til å bedre betydelig vår forståelse av den mørke energiens gåtefulle natur.

The Universe has entered an accelerating expansion phase in the last few billion years of its evolution, a phenomenon that is caused by the mysterious entity known as dark energy. Through the NEVO proposal, I aim to provide key insights about the properties of dark energy, which includes its time evolution and spatial distribution in the Universe. This objective will be achieved by utilizing cutting-edge cosmological N-body simulations and novel techniques to study cosmic EVOlution accurately.

A comprehensive understanding of the nature of dark energy requires a thorough investigation of viable candidates and their impact on different stages of the Universe. However, the consistent and accurate modeling of dark energy candidates has been largely neglected so far. To address this gap, I propose the development of a novel, integrated framework that utilizes state-of-the-art relativistic N-body simulations along with the hi_class code, to accurately model dark energy candidates in both linear and non-linear scales.

NEVO: illuminating the dark cosmos

Prosjektet «Wheelchair Activity Monitoring» (WHAM) sikter både på å øke fysisk aktivitet blant rullestolbrukere, og på å bidra til bredere mål om mangfold og inkludering av personer med nedsatt mobilitet, ved å fremme større tilgjengelighet og deltakelse gjennom innovativ teknologi og forskning.

Rullestolbrukere er tre til fem ganger mindre aktive enn den generelle befolkningen, noe som øker risikoen for å utvikle forebyggbare sykdommer. Selv om det finnes retningslinjer for fysisk aktivitet som er tilpasset rullestolbrukere, trenger vi fortsatt effektive måter å implementere disse på samt nøyaktig teknologi for å spore mulige endringer i fysisk aktivitet. WHAM har som mål å bidra til økt fysisk aktivitet blant rullestolbrukere ved å lage digitale verktøy for å monitorere og gi tilbakemelding på rullestolbrukerens aktivitet. WHAM består av tre faser: to år ved University of British Columbia (UBC), som inkluderer et samarbeid med eksperter innen atferdsendrings-intervensjoner for rullestolbrukere; ett år ved Norges teknisk-naturvitenskapelige universitet (NTNU) og et halvt år ved Maastricht Instruments - de to siste fasene er viet til å utvikle de digitale teknologiene. 

WHAMs mål er:
1)	Å teste effekten av et treningsprogram på fysisk aktivitetsnivå og fitness blant rullestolbrukere,
2)	Å utvikle nøyaktige algoritmer for monitorering av fysisk aktivitet blant rullestolbrukere,
3)	Å lage et system som både monitorerer og gir tilbakemelding på fysisk aktivitet blant rullestolbrukere.

Wheelchair users (WCU) are three to five times more inactive than the general population, leading to a high risk of developing preventable secondary conditions. Although physical activity (PA) guidelines have been specifically developed for WCU, we lack systematic approaches to implement them in practice, as well as valid algorithms to monitor PA change in WCU through sensor-based solutions. Accordingly, the overall scientific aim of the WHeelchair Activity Monitoring project (WHAM) is to advance PA research and practice in WCU by developing valid digital markers for monitoring of and providing feedback on PA, paving the way for implementation and intervention. I will accomplish this through combining the complementary research fields of 1) behavioral intervention studies focusing on increasing PA in WCU (core competence University of British Columbia, UBC; outgoing phase) and 2) sensor-based PA monitoring (core competence Norwegian University of Science and Technology, NTNU; returning phase – consolidated by non-academic placement at Maastricht Instruments, which develop research prototypes including PA monitors). WHAM’s scientific aim is achieved through three objectives: 1) Measure the effect of a pragmatic exercise intervention trial on self-reported physical activity levels and cardio-respiratory fitness, 2) Develop valid algorithms for tracking PA markers in WCU, and 3) Co-create
a framework that outlines how PA can be conjointly monitored and provided feedback on in WCU. This fellowship will enable me to become established as an internationally recognized researcher in the field of “physical activity and disability”, while bringing together ongoing national disjointed research activities in this field. Altogether, my overall ambition is to contribute to reducing inequalities in daily-life participation among WCU compared to the rest of the population.

Topfinancing - WHeelchair Activity Monitoring project (WHAM)

Distribuert Hydrogen Injeksjon og Forbrenning Teknologi for Neste Generasjons Pre-Combustion CCS

Midlene fra CLIMIT-Forskning, utvikling og demo av CO2-håndtering fordelt på år

Midlene fra CLIMIT-Forskning, utvikling og demo av CO2-håndtering fordelt på fylker

Midlene fra CLIMIT-Forskning, utvikling og demo av CO2-håndtering fordelt på fagområde

Seniorforsker

Final report about DiHI-Tech project progress and achievements.

The DiHI-Tech project aims to develop an innovative gas turbine burner design and fuel injection technology that is optimized to enable environmental-friendly (low NOx), energy- and cost-efficient (diluent-free fuel) premixed combustion of hydrogen for zero-emission power generation systems. This innovative fuel injection technology is based on the idea of substituting traditional nozzles (tiny millimetre-sized holes) for pointwise fuel injection in gas turbine burners with a completely new fuel injection system that is based on an opportunely shaped porous metal structure (micrometer-sized pores). The proposed concept effectively replaces conventional fuel injection nozzles, thereby eliminating the occurrence of concentrated point sources of the highly reactive hydrogen gas and providing instead its injection in a spatially distributed fashion, ultimately resulting in increased stability of the combustion system. Furthermore, earlier research conducted by SINTEF and DLR in the FME BIGCCS has already shown that porous steel also represents an effective acoustic barrier between flame and fuel system, ultimately resulting in a combustion system that is more resilient to thermo-acoustic instabilities.

All project objectives are successfully achieved in the project period.

1) The main outcome of the project is the confirmation that the DIHI-Tech concept for spatially distributed hydrogen injection in a model gas turbine combustor does work as expected also at gas turbine conditions, i.e. high pressure (10 atm) and reactants temperature (573 K) that are the operational limits of SINTEF Energy's HIPROX rig (rated at 100 kW of max thermal load). This is a significant achievement at lab scale and enables eventual further studies and demonstrations at higher TRL level and with the eventual inclusion of a gas turbine OEM. The final set of HIPROX experiments were concluded in December 2020 and January 2021 and a draft paper is produced for submission to the ASME TurboEXPO conference 2022 (submission opens in the summer 2021).

2) The work conducted at NTNU, both on the numerical modelling side and on the laboratory experiments side, has achieved the stated objectives of establishing the needed scaling laws for turbulent scalar mixing of light and fastly-diffusing gases (hydrogen or helium) injected into the main cross-flow of air through a porous surface. Three papers are already published in high-impact journals ("Fuel", "Experiments in Fluids" and "Journal of Fluid Mechanics") and a fourth draft is prepared for submission to the Journal of Fluid Mechanics.

3) The work conducted at SINTEF Industry on the testing and characterisation of hydrogen flow through the porous steel material, at the conditions encountered in the combustion system, has represented an optimal and required complement to both the combustion experiments conducted by SINTEF Energy and the scalar mixing experiments conducted by NTNU. A review paper on material science challenges encountered in hydrogen-fired gas turbines is submitted to the high-impact journal "International Materials Reviews" and is presently under review.

The increasing relevance of hydrogen-containing fuels in pre-combustion Carbon Capture and Storage (CCS) for power generation and in energy storage schemes means in practice that modern combustion systems must be able to use these new fuels efficiently while still complying with safety and emissions regulations. The DiHI-Tech project will develop an innovative gas turbine burner design and fuel injection technology that is optimized to enable environmental-friendly (low NOx), energy- and cost-efficient (diluent-free) premixed combustion of hydrogen-rich gaseous fuels. This innovative fuel injection technology is based on a spatially distributed injection system in which hydrogen enters the oxidant stream (typically air) through the surface of an aerodynamically-optimised porous structure. The proposed concept therefore replaces conventional fuel injection nozzles and eliminates the occurrence of concentrated point sources of the highly reactive hydrogen gas providing increased flame stability and reduced tendency to thermo-acoustic oscillations by the combustion system. This is a multi-disciplinary research project that builds upon promising preliminary results obtained within the framework of the (now ended) BIGCCS Research Centre and its main goal is to advance the proposed DiHI-Tech concept from the present Technical Readiness Level (TRL) 3 "Proof of Concept" to TRL 5 "Concept Validated in Relevant Environment". The successful achievement of this target will mature the technology and accelerate its industrial deployment that, in turn, will greatly reduce the economic and energetic cost of pre-combustion CCS schemes by eliminating the need for preparation (separation/pressurization) of large quantities of fuel diluents.

Distributed Hydrogen Injection and Combustion Technology for Next Generation Pre-Combustion CCS Schemes

Dei langkjeda omega-3 feittsyrene EPA og DHA er essensielle for menneske, og også for marine fiskeartar. I dag er viktigaste kjelda fiskeolje. Sidan det ikkje er råd å auke fangsten av villfisk, så er vidare vekst innan havbruk både i Noreg og globalt avgrensa av den tilgjengelege mengda av fiskeolje. Det trengst nye, berekraftige kjelder for EPA og DHA. Thraustochytridar er eincella eukaryotar, som kan akkumulere store mengder lipid, med eit høgt innhald av DHA. Dei kan dyrkast ved høge cellekonsentrasjonar og er svært lovande organismar for å utvikle konkurransedyktige produksjonsprosessar for omega-3 feittsyrer. Det har vore forska på thraustochytridar i mange år. Men sjølv om det er kjend at DHA og meitta feittsyrer vert syntetiserte via to konkurrerande spor så manglar det framleis ei grunnleggjande forståing av feittsyresyntesen i desse organismane. AurOmega-partnarane NTNU og SINTEF har dei siste tiåra isolert mange thraustochytridstammer og kartlagt evna deira til å lage lipid. 
I AurOmega nytta vi ein systembiologisk tilnærmingsmåte for betre å forstå kva som avgrensar DHA-syntesen i thraustochytridar og dermed korleis han kan aukast. Prosjektet nytta ein iterativ prosess der eksperimentelle disiplinar var tett integrerte med omfattande -omicsanalyser og matematisk modellering. Ein metabolsk modell vart laga basert på genomdata og kunnskap om vekst og sukkeropptak frå fermenteringa og validert med eksperimentelle data. Han kan nyttast til å føreseie kor mykje organismen kan produsere av ulike metabolittar og til kompleks nettverksanalyse for å identifisere nøkkelregulatorar for DHA-syntese, med eit særleg fokus på å auke synteseraten til DHA og inkorporering av DHA i lagringslipida. Seks massespektrometri-baserte metodar for kvantifisering av sentrale metabolittar er utvikla og optimaliserte for thraustochytridar. Desse metodane dekkjer sentralmetabolismen og gjev eit høgoppløyseleg bilete av metabolsk status under vekst og lipidakkumulering. Massespektrometri-basert metodikk for analyse av feittsyresamansetning, lipidklassefordeling og påvising av individuelle lipidmolekyl er også etablert. Desse metodane saman med proteomanalyser vart tekne i bruk for å analysere bioreaktorkultiveringar med høg-oppløyselege tidsserie-prøveuttak. Vi har også studert effekten av å inhibere spor som konkurrerer med DHA-syntesen på DHA syntese. Metodar for genomredigering er etablert og nytta til å studere ein feittsyredehydrogenase og to enzym involverte i danning av lagringslipid. Den nye kunnskapen vil kunne nyttast til å auke DHA-produktiviteten i utvalde thraustochytridestammer. Dette vil leggje grunnlaget for ein berekraftig og økonomisk levedyktig prosess for industriell omega-3 produksjon som kan mogleggjere vidare vekst for ein av dei viktigaste industriane i Noreg.

The long-chain omega-3 fatty acids EPA and DHA are essential for humans, as well as for marine fish species. The current source is fish oil. As wild fish catches cannot be further increased, continued growth of marine aquaculture, in Norway and globally, is now seriously constrained by the availability of fish oil.  New, sustainable sources of EPA and DHA are needed.  Thraustochytrids are unicellular eukaryotic microorganisms, able to accumulate high levels of lipids. They can be cultivated at high cell concentration and are extremely promising organisms for development of economic competitive production processes for omega-3 fatty acids.  Despite many years of research, there is still a lack of basic understanding of fatty acid synthesis in thraustochytrids, where DHA and saturated fatty acids are produced by two competing pathways. AurOmega partners NTNU and SINTEF have over the last decade isolated a high number of thraustochytrid strains and characterized their lipid-producing potential. The systems biology approach in AurOmega will provide an enhanced understanding of what limits the DHA synthesis in thraustochytrids and how it can be improved. An iterative approach applying high integration of experimental disciplines, with extensive omics analyses and mathematical modelling will be used. The mathematical and computational analysis will be based on genome-scale metabolic reconstruction and simulations to predict metabolic performance profiles, and complex network analysis to identify key regulatory features of DHA-synthesis, with particular focus of increasing the rate of DHA-synthesis and introduction into the storage lipids. The acquired new knowledge will be translated into enhanced DHA production capabilities of selected thraustochytrid strains. This will form the basis for a sustainable and economically feasible industrial omega-3 fatty acid production process, thereby enabling further growth of one of the most important industries in Norway.

DL: Microbial production of omega-3 fatty acids - a model based approach

Butanol er et attraktivt biodrivstoff og som også tillater bruk av eksisterende infrastruktur og kjøretøy. Produksjon av butanol er begrenset av energikrav i syntesen fra etanol og med lave utbytter. Eksisterende biologiske prosesser for produksjon av butanol har lav effektivitet. Acetaldehyd er et mellomprodukt i syntesen av butanol men produksjon av acetaldehyd er kostbart. Mikroorganismer kan brukes for produksjon av acetaldehyd. Bakterien Zymomonas mobilis har den raskeste etanolsyntesen og høyeste etanolutbytte av alle kjente etanolproduserende mikroorganismer. Acetaldehyd er det siste mellomproduktet i syntese av etanol, og for å beholde red-oks balansen i cellen må reduserende ekvivalenter fjernes hvis man skal inhibere det siste metabolske trinnet mellom acetaldehyd og etanol. Z. mobilis har en aktiv respiratorisk kjede med lavt energiutbytte, og respirasjon kan brukes til å fjerne reduserende ekvivalenter i en ellers fermentativ prosess. Derfor kan Z. mobilis respirasjonskjeden brukes til å balansere red-oks balansen i en acetaldehyd-produserende Z. mobilis bioprosess. Produktiviteter og utbytter vil være begrenset av metabolsk inhibering forårsaket av acetaldehyd som er sterkt inhiberende ved lave konsentrasjoner. Kontinuerlig fjerning av acetaldehyd er derfor nødvendig for å kunne etablere en effektiv og konkurransedyktig bioprosess. Konseptet bak Z-Fuels er å designe, lage og teste ut en bakteriell fermenteringsprosess med en integrert mikroboble destilleringssystem som muliggjør kontinuerlig fjerning av acetaldehyd.

Butanol is a desirable biofuel as its characteristics most closely match gasoline and allows using current gasoline fuel infrastruture and vehicles. Its production however is limited by the energy requirements needed for its synthesis from ethanol and the poor yields. A known intermediary in butanol production is acetaldehyde, the production of which involves high cost. Microorganisms exist which can produce acetaldehyde directly. The bacterium Zymomonas mobilis is the most prominent example, which bears a great potential for bioprocess improvement by metabolic engineering. However, the process yields and productivity are restricted by the metabolic inhibition caused by the produced acetaldehyde. The concept of the present project is to design, construct and operate a bacterial process, based on genetically engineered Z. mobilis with an integrated microbubble distillation system to convert complex sugary feedstocks and crude glycerol to acetaldehyde. Effective removal of acetaldehyde during the metabolic process will alleviate the inhibition and give higher yields. From the higher production quantities of acetaldehyde a more competitive and efficient route to butanol production can be obtained compared to current practise.

ERA-NET: Z-Fuels - A novel bacterial system with integrated micro-bubble distillation for the production of acetaldehyde

Forskningsprosjektet TERPENOSOME - Engineered compartments for monoterpenoid production using synthetic biology skal bruke syntetisk biologi teknologi for å etablere overproduksjon av monoterpenoider i de mikrobielle vertsorganismene E. coli og S. cerevisiae. Produksjonen skal skje i kunstige indre organeller i vertsorganismen for å redusere syntesen av metabolske sideprodukter og eliminere toksisitets-problematikken som begrenser produktiviteten i eksisterende prosesser. Organellene vil bli basert peroksisomer i S. cerevisiae og protein-baserte i E. coli. Et hovedmål er å oppnå høyere og mer effektiv produksjon enn i planter, spesielt for høykostnads terpenoider. TERPENOSOME har som mål å: 1) etablere en portefolio av generiske metoder for konstruksjon av metabolske spor i kunstige organeller; 2) forbedre metabolske enzym-systemer for mer effektiv produksjon av kommersielt interessante metabolitter; 3) overprodusere aktuelle terpenoider for mulig kommersialisering. Den generiske kunstige organelle- teknologien vil kunne bli utnyttet av den ene industripartneren og derfor bli tilgjengelig for syntetisk biologi prosjekter for en rekke bioteknologiske produkter. Den andre industripartneren vil kunne utnytte forbedrede produksjonsstammer for terpenoidet limonene og to andre kommersielt interessante produkter.

The main objective of TERPENOSOME is to use synthetic biology to engineer novel organelles for the overproduction of monoterpenoids in microbial hosts. We intend to achieve higher and more efficient production than is seen naturally in plants. Compartment alization will eliminate the toxicity problems that currently hinder higher productivity and will also reduce side products. The engineered organelles will be either natural (peroxisomes in yeast) or artificial (protein-based compartments in Escherichia c oli).

TERPENOSOME expected results:
1) Generate a portfolio of generic methods for the compartmentalization of biosynthetic pathways for small bioactive molecules, especially terpenoids;
2) Improve the biosynthetic enzyme systems of terpenoid biosynthesi s for new and more efficient bioprocessing;
3) Overproduce an industrially relevant terpenoid, which will be commercialized by one of the partners.

Exploitation:
The potential for industrial application is extremely high for two of the main results. 
Fir st, the compartmentalization methodology we establish will be generic and can be applied to a wide range of biotechnologically relevant high-value compounds. The participation of Life Technologies in the consortium will accelerate the time-to-market for a ny of these methodological advances and the resulting improvements in compound production.  
Second, in addition to the broadly used precursor material limonene, the major target molecules of the project are two high-value compounds of commercial interest  to the second industry partner, ACS International.

0 projects

BIOTEK2021-Bioteknologi for verdiskaping

DL: Microbial production of omega-3 fatty acids - a model based approach

Awarded: NOK 20.0 mill.

Project Period: 2017-2021

Location: Trøndelag - Trööndelage

BIOTEK2021-Bioteknologi for verdiskaping

ERA-NET: Z-Fuels - A novel bacterial system with integrated micro-bubble distillation for the production of acetaldehyde

Awarded: NOK 4.3 mill.

Project Period: 2016-2019

Location: Trøndelag - Trööndelage

BIOTEK2021-Bioteknologi for verdiskaping

TERPENOSOME: Engineered compartments for monoterpenoid production using synthetic biology

Awarded: NOK 3.6 mill.

Project Period: 2014-2017

Location: Trøndelag - Trööndelage