Regulation of Nuclear Receptor Function by Posttranslational Modifications

Nuclear receptors (NRs), together with coregulatory proteins, coordinate the transcription of gene networks in response to ligand binding and cellular signaling in such diverse biological programs as reproduction, development, nutritent uptake, metabolism and excretion. PMTs have proven to be essential for NR and cofactor activity, although the molecular details are generally sparse. The possibility to analyze modified peptides in a large-scale manner and to create PTM maps of selected proteins is fundame ntal to obtain a comprehensive picture of protein (e.g. NRs) function. In accordance with the call for FUGEII proposals, the project described here will contribute to solve this methodological issue, and when established the method will considerably incre ase the service provided by the FUGE proteomic platform (PROBE). Also, new insights into the biology of NRs will be revealed, -information that are of relevance for designing novel therapies.