The potential for pain perception in teleost fish is a controversial issue and represents a focal point for the fundamental understanding of fish welfare. In this proposal, our main objective is to determine which brain areas are involved in the detection of noxious stimuli, thus specifically targeting the most central elements of the current scientific debate on pain in fish. This will be achieved through an interdisciplinary approach, combining methodology such as neurophysiology, immunohistochemistry a nd functional magnetic resonance imaging (fMRI).