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HAVBRUKS-Havbruk - en næring i vekst
The role of novel interferons in antiviral immunity and DNA vaccine mechanism in Atlantic salmon
Awarded: NOK 3.0 mill.
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Project Number:
185248
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Project Period:
2008 - 2012
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To develop more efficient vaccines and other methods to fight viral diseases of Atlantic salmon there is a need for a better understanding of antiviral immune mechanisms in this species. Interferons (IFNs) play a crucial role in innate immune responses ag ainst virus infections in mammals whereby host cells produce and secrete IFNs upon recognition of viral nucleic acids. These IFNs protect other cells from further viral infection by inducing IFN stimulated genes (ISGs), several of which encode antiviral p roteins such as Mx and ISG15. Research in our laboratory has contributed to characterization of the innate IFN system of Atlantic salmon. Two IFNs (IFN-a1 and IFN-a2) have previously been cloned from salmon and characterized with respect to gene structure , promoter antiviral activity and effect on expression of ISGs. We have recently cloned two novel IFNs from Atlantic salmon, which we have preliminary named IFNb and IFNc. In this project we first want to explore expression properties and antiviral activi ty of the novel IFNs. In mammals, innate IFNs also have an important role in mounting an effective adaptive immune response. We now want to explore if innate IFNs (IFN-a1, IFNb and IFNc) stimulate the adaptive immune responses in salmon by using DNA vacci ne as a model system. The most efficient vaccines against viral diseases in fish to date at the experimental level are DNA vaccines against the salmonid rhabdoviruses VHSV and IHNV. These vaccines are based intramuscular injection of plasmids encoding the viral G-gene. Innate IFNs are hypothesised to play a role both in the early non-specific and the long lasting specific antiviral protection in the fish by these vaccines. In this project we want to compare the induction of ISGs by i.m. injected IFN-genes , IHNV G-gene, ISAV hemagglutininesterase gene and IPNV polyprotein gene. Moreover, we will study if the IHNV G-gene or the IFN-genes may have co-stimulatory effects in DNA vaccination against ISAV and IPNV.
Funding scheme:
HAVBRUKS-Havbruk - en næring i vekst
1.3BILL. NOKtotal funding in the programme period511PROJECTShave received funding in the programme period7SOURCEShave financed the programme
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Globale utfordringerGlobal matsikkerhetBransjer og næringerFiskeri og havbrukPortefølje ForskningssystemetPolitikk- og forvaltningsområderNordområdeneMarinGlobale utfordringerLTP3 Hav og kystMatGlobal matsikkerhetLTP3 Styrket konkurransekraft og innovasjonsevneLTP3 Fagmiljøer og talenterInternasjonaliseringInternasjonalt prosjektsamarbeidLTP3 Nano-, bioteknologi og teknologikonvergensMatMarinHavbrukLTP3 Samfunnssikkerhet og beredskapNordområdeneMiljø og marine ressurser (utgår 2010)LTP3 Marine bioressurser og havforvaltningLTP3 Samfunnsikkerhet, sårbarhet og konfliktLTP3 Et kunnskapsintensivt næringsliv i hele landetMatMat - Blå sektorPolitikk- og forvaltningsområderFiskeri og kystBioteknologiGrunnforskningInternasjonaliseringBransjer og næringerPortefølje Banebrytende forskningLTP3 Høy kvalitet og tilgjengelighetLTP3 Muliggjørende og industrielle teknologierPortefølje Mat og bioressurserPortefølje Muliggjørende teknologier