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Androgens and growth of previtellogenic oocytes in Atlantic cod: Identification and patterns of differentially expressed genes and proteins

Tildelt: kr 73 999

Despite the concept of life-history strategies, the internal (within the body) signals that regulate fecundity are not known. Prior to vitellogenesis (previtellogenesis), androgens may play an integral role in the regulation of oocytes (immature eggs) gro wth. Preliminary evidence supporting these assumptions are derived from the induction of oocyte growth in in vitro androgen exposed immature female eels, probably by gene regulation mechanisms (genomics) or by protein modification mechanisms (proteomics). In this study we will use the analytical power of SSH to open a possible revealing window of understanding into the functional aspects of oocyte development in organisms that control fecundity. By explaining how fecundity is controlled in teleosts, these results will have some positive implications in the aquaculture industry (particularly for new species) and in fisheries management (especially for declining fish stocks such as the Atlantic cod, G. morhua). The overall objective of the proposed project is to study the role/effect of androgens (e.g. 11-ketotestosterone; 11-KT) on the growth of previtellogenic oocytes through the characterization of androgen-responsive ovarian genes and proteins. Thus, increasing our understanding about hormonal control o f previtellogenic oocyte growth in teleosts. To assess the utility of suppression subtractive hybridization (SSH) as a method of gene discovery in the previtellogenic ovarian oocytes of teleosts, we have sequenced 265 subtracted gene clones from two cDNA libraries constructed from the mRNA of previtellogenic ovarian oocytes, before and after exposure to 11-KT, methytestosterone and testosterone. A gene array was recently printed at the FUGE microarray technology platform at Medical Technical Center (MTS) NTNU. This oversea fellowship is for PhD candidate Trond M. Kortner to perfrom the projected travel to our collaborators in Portugal and do analysis to identify cell types expressing the sequenced genes.

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HAVBRUKS-Havbruk - en næring i vekst

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